Research in the Seyfried lab is focused on the integration of proteomics, systems biology, and molecular biology to tackle fundamental questions related to the pathogenesis of Alzheimer’s Disease (AD) and other neurodegenerative disorders. In particular, we utilize high resolution liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to identify and quantify proteins and post-translational modifications (PTMs). Combining tools in both molecular and cellular biology, we also explore the relationship between the function of these proteins and their PTMs in the development of these devastating diseases.
Our funded projects are currently focused on the development of mass spectrometry-based techniques for:
(1) Systems level analysis of quantitative proteomic and transcriptomic expression in human donor postmortem brain tissue
(2) Identification and quantification of protein post-translational modifications including ubiquitination, phosphorylation, methylation and acetylation
(3) Proteomic biomarker discovery in cerebrospinal fluid (CSF), plasma and platelets in Alzheimer’s Disease and other related neurodegenerative diseases
(4) Proteogenomic applications to identify rare coding changes at the peptide level in AD candidate risk genes
November 2017, Paper Accepted: Mfon’s paper titled, “A proteomic network approach across the ALS-FTD disease spectrum resolves clinical phenotypes and genetic vulnerability in human brain,” was accepted for publication to the EMBO Molecular Medicine. This work was done in collaboration with Jonathan Glass (Emory Neurology), Director of the Emory ALS Clinic.
September 2017, Paper Accepted: Ian’s paper titled, “Generation of Clickable Pittsburgh Compound B for the Detection and Capture of β-Amyloid in Alzheimer's Disease Brain” was accepted for publication at Bioconjugate Chemistry. This work was done in collaboration with Lary Walker (Emory Neurology).
August 2017, Press Release: Excellent summary of our new GenPro software by Genomeweb, “Emory Team Develops New Software for Building Sample-Specific Mass Spec Reference Databases”.
August 2017, Paper Review: GenPro is highlighted on Ben Orsburn’s blog, “GenPro -- 1 step closer to personalized proteomics!”.
July 2017, Press Release: Our research on Alzheimer’s Disease protein networks was highlighted in an article on Alzforum titled, "Alzheimer’s Proteomics Treasure Trove?"