• The Seyfried Lab

Contact

Nicholas T. Seyfried, PhD

Assistant Professor, Department of Biochemistry
Director, Emory Integrated Proteomics Core
Emory University School of Medicine

(404) 712-8589
nseyfri@emory.edu

Research in the Seyfried lab is focused on the integration of proteomics, systems biology, and molecular biology to tackle fundamental questions related to the pathogenesis of Alzheimer’s Disease (AD) and other neurodegenerative disorders. In particular, we utilize high resolution liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to identify and quantify proteins and post-translational modifications (PTMs). Combining tools in both molecular and cellular biology, we also explore the relationship between the function of these proteins and their PTMs in the development of these devastating diseases.

Our funded projects are currently focused on the development of mass spectrometry-based techniques for:

(1) Systems level analysis of quantitative proteomic and transcriptomic expression in human donor postmortem brain tissue
(2) Identification and quantification of protein post-translational modifications including ubiquitination, phosphorylation, methylation and acetylation
(3) Proteomic biomarker discovery in cerebrospinal fluid (CSF), plasma and platelets in Alzheimer’s Disease and other related neurodegenerative diseases
(4) Proteogenomic applications to identify rare coding changes at the peptide level in AD candidate risk genes

Latest News

May 2018, Thesis Defense: BCDB graduate student Isaac Bishof successfully defended his PhD thesis. Congrats Dr. Bishof!

May 2018, paper accepted: Isaac’s paper titled “RNA-binding proteins with basic-acidic dipeptide (BAD) domains self-assemble and aggregate in Alzheimer's disease” was recently accepted for publication by the Journal of Biological Chemistry.

March, 2018, conference presentation: Nick presented a talk titled “Integrative Proteomics for Novel Target and Biomarker Discovery in Alzheimer’s Disease” at the National Institutes of Health (NIH) Alzheimer’s Disease Research Summit in Bethesda Maryland. Click link above to see Nick’s talk at the 4:21:00 mark.

March 2018, paper accepted: Lingyan’s paper titled “Global quantitative analysis of the human brain proteome in Alzheimer's and Parkinson's Disease” was recently accepted for publication by the Scientific Data.

Funding

ALZ Assoc
MJFF
CND
NINDS
NIA NIH