Research in the Seyfried lab is focused on the integration of proteomics, systems biology, and molecular biology to tackle fundamental questions related to the pathogenesis of Alzheimer’s Disease (AD) and other neurodegenerative disorders. In particular, we utilize high resolution liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to identify and quantify proteins and post-translational modifications (PTMs). Combining tools in both molecular and cellular biology, we also explore the relationship between the function of these proteins and their PTMs in the development of these devastating diseases.
Our funded projects are currently focused on the development of mass spectrometry-based techniques for:
(1) Systems level analysis of quantitative proteomic and transcriptomic expression in human donor postmortem brain tissue
(2) Identification and quantification of protein post-translational modifications including ubiquitination, phosphorylation, methylation and acetylation
(3) Proteomic biomarker discovery in cerebrospinal fluid (CSF), plasma and platelets in Alzheimer’s Disease and other related neurodegenerative diseases
(4) Proteogenomic applications to identify rare coding changes at the peptide level in AD candidate risk genes
In the News
June 2017, Conference Presentation: Nick Presented at talk titled “Protein co-expression network analysis of asymptomatic Alzheimer’s disease” at the EMBO | EMBL Symposia on Mechanisms in Neurodegeneration in Heidelberg Germany.
March 2017, Conference Presentation: Nick Presented at talk titled “A Multi-Network Approach to Define Pathways Altered in Alzheimer’s Disease and Parkinson’s Disease” at the US HUPO meeting in San Diego, CA
March 2017, Travel Award: Congratulations to Isaac Bishof who was awarded a Travel Grant for his attendance at the 2017 RNA Society meeting in Prague to present a poster titled “A Network Approach Reveals Novel Roles of Low Complexity Domains in Spliceosomal Proteins”
January 2017, Grant News: Nick, Dean Jones and Eric Ortlund were awarded a $13.5 grant from the NIH to serve as a site for the Molecular Transducers of Physical Activity in Humans Program. This work will also be conducted in collaboration with GaTech. See link.