Publications

Francis JWGoswami DNovick SJPascal BDWeikum EROrtlund EAGriffin PRKahn RA, 2017. Nucleotide binding to ARL2 in the TBCD-ARL2-beta-tubulin complex drives conformation changes in beta-tubulin.  J Mol Bio. 

Weikum, E.R., S. de Vera, I.M., Nwachukwu, J.C., Nettles, K.W., Kojetin, D. K., Ortlund E.A., 2017, The glucocorticoid receptor binds directly to GR half-site sequences embedded within AP-1 recognition motifs, Nucleic Acids Research. 

Weikum ER, Okafor DC, D'Agostino EH, Colucci JK, Ortlund EA, 2017. Structural analysis of the glucocorticoid receptor ligand-binding domain in complex with triamcinolone acetonide and a fragment of the atypical coregulator, SHP. Molecular Pharmacology.

Mays SG, Okafor CD, Tuntland ML, Whitby RJ, Dharmarajan V, Stec J,  Griffin PR, Ortlund EA. 2017. Structure and Dynamics of the Liver Receptor Homolog 1-PGC1α Complex. Molecular Pharmacology.

Weikum ER, Knuesel MT, Ortlund EA, Yamamoto KR. 2017. Glucocorticoid receptor control of transcription: precision and plasticity via allostery. Nature Reviews Molecular Cell Biology.

Weikum, E.R., Knuesel, M.T., Ortlund E.A.*, Yamamoto, K.R.*, 2016, Invited Review: Control of transcription by the glucocorticoid receptor: Precision, plasticity, and allostery. Nature Reviews Molecular Cell Biology.

Weikum E.R., Tuntland, M.L., Murphy, M.N., Ortlund, E.A., Structure of GCNF and LRH-1 DNA Binding domains in complex with Oct4 promoter reveals overlapping binding sites., Journal of Molecular Biology 

Mays, S.G., Okafor, C.D., Whitby, R.J., Goswami, D., Stec, J., Flynn, A.R., Dugan, M.C., Jui, N.T., Griffin, P.R., Ortlund, E.A. Crystal Structures of LRH-1 with Synthetic Agonists Reveal a Novel Mechanism of Receptor Activation.   Journal of Biological ChemistryAOP *JBC paper of the week and Cover Image

Hudson W.H., Kossmann, B.R., S. de Vera, I.M., Chuo, S., Weikum, E.R., Eick, G., Thornton, J.W.,  Ivanov, I., Kojetin, D. K., Ortlund E.A., Distal substitutions drive divergent DNA specificity among paralogous transcription factors through a subdivision of conformational space, Proceedings of the National Academy of Sciences 113 (2), 326-331

PM Musille, BR Kossmann, JA Kohn, I Ivanov, EA Ortlund. Unexpected Allosteric Network Contributes to LRH-1 Co-regulator Selectivity. Journal of Biological Chemistry 291 (3), 1411-1426

Yu, K., Whitlock, J.M., Lee, K., Ortlund, E.A., Yui, C. C., Hartzell, C., Identification of a Lipid Scrambling Domain in ANO6/TMEM16F, eLIFE. 4:e06901.

Brown, N.E., Goswami, D., Branch, M.R., Ramineni, S., Ortlund, E.A., Griffin, P.R., Hepler, J.R., Integration of G Protein Alpha (Gα) Signaling by the Regulator of G Protein   Signaling 14., Journal of Biological Chemistry. jbc.M114.634329.

Folick A, Oakley HD, Yu Y, Armstrong EH, Kumari M, Sanor L, Moore DD, Ortlund EA, Zechner R, Wang MC., Aging. Lysosomal signaling molecules regulate longevity in Caenorhabditis eligans., Science.

Qiang G., Xue, S., Yang J.J., Du, G., Pang X., Li X., Goswami D., Griffin P.R., Ortlund E.A., Chan C.B., Ye K., Identification of a Small Molecular Insulin Receptor Agonist with Potent Anti-diabetic Activity. Diabetes.
Hudson W.H., Ortlund E.A., Analysis: The dual nature of DNA/ RNA binding proteins. Nature Reviews Molecular Biology.
Hudson W.H., Ortlund E.A., X-ray crystal structure of the mineralocorticoid receptor DNA binding domain – DNA complex. PLoS One. 
Armstrong, E.H., Noy, N., Ortlund, E.A. Structural basis for ligand regulation of the FABP5-PPARβ/δ signaling pathway. JBC 
Ren, J., Pathak, M.C., Temple, B.R.S., Lin, C., Nile, A.H., Mousley, C.J., Duncan, M.C., Eckert, D.M., Leiker, T.J., Ivanova, P.T., Meyers, M.S., Murphy, R.C., Brown, H.A., Verdaasdonk, J., Bloom, K.S., Ortlund, E.A., Neiman, A.M., Bankaitis, V.A. A Phosphatidylinositol Transfer Protein Integrates Phosphoinositide Signaling With Lipid Droplet Metabolism To Regulate a Developmental Program of Nutrient Stress-Induced Membrane Biogenesis. Mol Cell Bio.
Kratzer, J.T., Miguel, L.A., Murphy, M.N., Cicerchi, C., Graves, C.L., Tipton, P.A., Ortlund, E.A.*, Johnson, R.J.*, Gaucher, E.A.* Evolutionary history and metabolic insights of ancient mammalian uricases., Proc Natl Acad Sci U S A
Musille, P.M and Ortlund E.A. The structure determination of glycerol dehydrogenase from Serratia. Acta Crystallogr F Struct Biol Cryst Commun.

Bridgham, J.T., Keay, J, Ortlund, E.A. and Thornton, J.W. Vestigialization of an allosteric switch:  Genetic and structural mechanisms for the evolution of constitutive activity in the steroid receptor. PLoS Genetics 

Hudson WH, Pickard MR, de Vera IM, Kuiper EG, Mourtada-Maarabouni M, Conn GL, Kojetin DJ, Williams GT, Ortlund EA.Conserved sequence-specific lincRNA-steroid receptor interactions drive transcriptional repression and direct cell fate. Nature Communications

Brent  M. Horton, William H. Hudson, Eric A. Ortlund, Sandra Shirk, James W. Thomas, Emily R. Young, Wendy Zinzow-Kramer, and Donna L. Maney. Promoter Polymorphism and Differential Expression of Estrogen Receptor α in a Species with Alternative Behavioral Phenotypes. Proc Natl Acad Sci (2013).
Michael J. Harms, Geeta N. Eick, Dev Goswami, Jennifer K. Colucci, Patrick R. Griffin, Eric A. Ortlund, Joseph W. Thornton. Biophysical mechanisms for large-effect mutations in the evolution of sterid hormone receptors. Proc Natl Acad Sci [PMID: 23798447]

Paul M. Musille, Manish C. Pathak, Jeni L. Lauer, Pattrick R. Griffin, Eric A. Ortlund. Divergent sequence tunes ligand sensitivity in Phospholipid-regulated hormone receptors. JBC. [PMID: 23737522]

Colucci JK, Ortlund EA. X-ray crystal structure of the ancestral 3-ketosteroid receptor-progesterone-mifepristone complex shows mifepristone bound at the coactivator binding interface.PLoS One. [PMID: 24260475]

Paul M. Musille, Jeffrey A. Kohn, Eric A. Ortlund. Phospholipid-driven gene regulation. FEBS Letters [PMID: 23333623]
William H. Hudson, Christine Youn, Eric A. Ortlund.  The structural basis of directl glucocorticoid-mediated transrepression. Nature Structural & Molecular Biology. [PMID: 23222642]

2012

Geeta N. Elick, Jennifer K. Colucci, Michael J. Harms, Eric A. Ortlund, Joseph W. Thornton. Evolution of minimal specificity and promiscuity in steroid hormone receptors.  Plos Genetics 8(11) [PMID:23166518]

Paul M. Musille, Manish Pathak, Janelle L. Lauer, William H. Hudson, Patrick R. Griffin, Eric A. Ortlund.  Antidiabetic Phospholipid – Nuclear Receptor Complex Reveals the Mechanism for Phospholipid Driven Gene Regulation. Nature Structural & Molecular Biology 19, 532-7 (2012). [PMID: 22504882]

Jeffrey A. Kohn, Kirti Desphande, Eric A. Ortlund.  Deciphering modern glucocorticoid cross-pharmacology using ancestral corticosteroid receptors. Journal of Biological Chemistry 20, 16267-75. [PMID: 22437833]

2011

Jae Man Lee, Yoon Kwang Lee Jennifer L. Mamrosh, Manish Pathak, Eric A. Ortlund, David D. Moore.  Antidiabetic Effects of a Novel Agonist Ligand for the Nuclear Receptor LRH-1. Nature 474, 506-U135. [PMID: 21614002]

Paul T. R. Thiruchelvam, Chun-Fui Lai, Hui Hua, Ross S. Thomas, Antoni Hurtado, William Hudson, Andrew R. Bayly, Fiona J. Kyle, Manikandan Periyasamy, Andrew Photiou, Alan C. Spivey, Eric A. Ortlund, Richard J. Whitby, Jason S. Carroll, R. Charles Coombes, Laki Buluwela, Simak, Ali. The liver receptor homolog-1 regulates estrogen receptor expression in breast cancer cells. Breast Cancer Research and Treatment 127, 385-396. [PMID: 20607599]

Gabriel Schaaf, Marek Dynowski, Carl J. Mousley, Sweety D. Shah, Peihua Yuan, Eva M. Winklbauer, Marilia K. F. de Campos, Kyle Trettin, Mary-Chely Quinones, Tatyana I. Smirnova, Lora L. Yanagisawa, Eric A. Ortlund*, Vytas A. Bankaitis*. Resurrection of a Functional Phosphatidylinositol Transfer Protein from a Pseudo-Sec14 Scaffold by Directed Evolution. Molecular Biology of the Cell 22, 892-905. [PMID: 21248202].

Jihui Ren, Gabriel Schaaf, Vytas Bankatis, Manish Pathak, Eric A. Ortlund.  Crystallization and preliminary X-ray diffraction analysis of Sfh3, a member of the Sec14 protein superfamily. Acta Crystallographica Section F 67, 1239-1243. [PMID: 22102037]

Sean M. Carroll, Eric A. Ortlund*, Joseph W. Thornton*. Mechanisms for the evolution of a derived function in the ancestral glucocorticoid receptor.  Plos Genetics 7. [PMID:21698144]

2010

Jamie T. Bridgham, Geeta N. Eick, Claire Larroux, Kirti Deshpande, Michael J. Harms, Marie E. A. Gauthier, Eric A. Ortlund, Bernard M. Degnan, Joseph W. Thornton. Protein evolution by molecular tinkering: diversification of the nuclear receptor superfamily from a ligand-dependent ancestor. Plos Biology 8. [PMID: 20957188]

Fei Chen, Eric A Gaucher, Nicole A Leal, Daniel Hutter, Stephanie A Havemann, Sridhar Govindarajan, Eric Ortlund, Steven Benner.  Reconstructed Evolutionary Adaptive Paths (REAP) give Polymerases Accepting Reversible Terminators for Sequencing and SNP Detection. Proceedings of the National Academy of Sciences of the United States of America 107, 1948-1953. [PMID: 20080675]

2009

Jamie Bridgham, Eric Ortlund, Joseph Thornton.  An Epistatic Ratchet Constrains the Direction of Glucocorticoid Receptor Evolution.  Nature 461, 515-U78. [PMID: 19779450]

          

2008


Eric Ortlund*, Gabriel Schaaf*, Kimberly R. Tyeryar, Carl J. Mousley, Kristina E. Ile, Teresa A. Garrett, Jihui Ren, Melissa J. Woolls, Christian R.H. Raetz, Matthew R. Redinbo, Vytas A. Bankaitis.  Functional Anatomy of Phospholipid Binding and Regulation of Phosphoinositide Homeostasis by Members of the Sec14 Protein Superfamily. Molecular Cell 29, 191-206. [PMID: 18243114]