In eukaryotic cells, the facilitated movement of macromolecules
through the nuclear pore complex is an essential step in gene
regulation. Transcription factors are imported into the nucleus while
the final products of transcription, mRNA transcripts, are exported to
the cytoplasm. The Corbett laboratory seeks to understand how these
transport events contribute to the regulation of gene expression and
how defects in these processes lead to human diseases such as mental
retardation and muscular dystrophy. To seek these answers, we employ
a large number of diverse approaches including genetic methods that
exploit the budding yeast, Saccharomyces cerevisiae, cell biological
methods, systems approaches, and biochemical and structural methods.
In addition to budding yeast, we use cultured patient cells,
Drosophila models, and transgenic mice.