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Strassler SE, Bowles IE, Krishnamohan A, Kim H, Edgington CB, Kuiper EG, Hancock CJ, Comstock LR, Jackman JE, Conn GL. tRNA m(1)G9 modification depends on substrate-specific RNA conformational changes induced by the methyltransferase Trm10. J Biol Chem. 2023 Dec;299(12):105443. doi: 10.1016/j.jbc.2023.105443. Epub 2023 Nov 8. PubMed PMID: 37949221; PubMed Central PMCID: PMC10704376.
Kavanaugh LG, Mahoney AR, Dey D, Wuest WM, Conn GL. Di-berberine conjugates as chemical probes of Pseudomonas aeruginosa MexXY-OprM efflux function and inhibition. bioRxiv. 2023 Sep 19;. doi: 10.1101/2023.03.24.533986. PubMed PMID: 37425949; PubMed Central PMCID: PMC10327050.
Srinivas P, Nosrati M, Zelinskaya N, Dey D, Comstock LR, Dunham CM, Conn GL. 30S subunit recognition and G1405 modification by the aminoglycoside-resistance 16S ribosomal RNA methyltransferase RmtC. Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304128120. doi: 10.1073/pnas.2304128120. Epub 2023 Jun 12. PubMed PMID: 37307464; PubMed Central PMCID: PMC10288597.
Strassler SE, Bowles IE, Dey D, Jackman JE, Conn GL. Tied up in knots: Untangling substrate recognition by the SPOUT methyltransferases. J Biol Chem. 2022 Oct;298(10):102393. doi: 10.1016/j.jbc.2022.102393. Epub 2022 Aug 18. Review. PubMed PMID: 35988649; PubMed Central PMCID: PMC9508554.
Laughlin ZT, Conn GL. Tuberactinomycin antibiotics: Biosynthesis, anti-mycobacterial action, and mechanisms of resistance. Front Microbiol. 2022;13:961921. doi: 10.3389/fmicb.2022.961921. eCollection 2022. Review. PubMed PMID: 36033858; PubMed Central PMCID: PMC9403184.
Laughlin ZT, Nandi S, Dey D, Zelinskaya N, Witek MA, Srinivas P, Nguyen HA, Kuiper EG, Comstock LR, Dunham CM, Conn GL. 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA. Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2120352119. doi: 10.1073/pnas.2120352119. Epub 2022 Mar 31. PubMed PMID: 35357969; PubMed Central PMCID: PMC9168844.
Schwartz SL, Dey D, Tanquary J, Bair CR, Lowen AC, Conn GL. Role of helical structure and dynamics in oligoadenylate synthetase 1 (OAS1) mismatch tolerance and activation by short dsRNAs. Proc Natl Acad Sci U S A. 2022 Jan 18;119(3). doi: 10.1073/pnas.2107111119. PubMed PMID: 35017296; PubMed Central PMCID: PMC8784149.
Dey D, Ramakumar S, Conn GL. Targeted Redesign of Suramin Analogs for Novel Antimicrobial Lead Development. J Chem Inf Model. 2021 Sep 27;61(9):4442-4454. doi: 10.1021/acs.jcim.1c00578. Epub 2021 Sep 13. PubMed PMID: 34516120.
Magg T, Okano T, Koenig LM, Boehmer DFR, Schwartz SL, Inoue K, Heimall J, Licciardi F, Ley-Zaporozhan J, Ferdman RM, Caballero-Oteyza A, Park EN, Calderon BM, Dey D, Kanegane H, Cho K, Montin D, Reiter K, Griese M, Albert MH, Rohlfs M, Gray P, Walz C, Conn GL, Sullivan KE, Klein C, Morio T, Hauck F. Heterozygous OAS1 gain-of-function variants cause an autoinflammatory immunodeficiency. Sci Immunol. 2021 Jun 18;6(60). doi: 10.1126/sciimmunol.abf9564. PubMed PMID: 34145065; PubMed Central PMCID: PMC8392508.
Schwartz SL, Park EN, Vachon VK, Danzy S, Lowen AC, Conn GL. Human OAS1 activation is highly dependent on both RNA sequence and context of activating RNA motifs. Nucleic Acids Res. 2020 Jul 27;48(13):7520-7531. doi: 10.1093/nar/gkaa513. PubMed PMID: 32678884; PubMed Central PMCID: PMC7367156.
Dey D, Kavanaugh LG, Conn GL. Antibiotic Substrate Selectivity of Pseudomonas aeruginosa MexY and MexB Efflux Systems Is Determined by a Goldilocks Affinity. Antimicrob Agents Chemother. 2020 Jul 22;64(8). doi: 10.1128/AAC.00496-20. Print 2020 Jul 22. PubMed PMID: 32457110; PubMed Central PMCID: PMC7526836.
Welty R, Rau M, Pabit S, Dunstan MS, Conn GL, Pollack L, Hall KB. Ribosomal Protein L11 Selectively Stabilizes a Tertiary Structure of the GTPase Center rRNA Domain. J Mol Biol. 2020 Feb 14;432(4):991-1007. doi: 10.1016/j.jmb.2019.12.010. Epub 2019 Dec 24. PubMed PMID: 31874150; PubMed Central PMCID: PMC7325582.
Kuiper EG, Dey D, LaMore PA, Owings JP, Prezioso SM, Goldberg JB, Conn GL. Substrate recognition by the Pseudomonas aeruginosa EF-Tu-modifying methyltransferase EftM. J Biol Chem. 2019 Dec 27;294(52):20109-20121. doi: 10.1074/jbc.RA119.011213. Epub 2019 Nov 21. PubMed PMID: 31753919; PubMed Central PMCID: PMC6937583.
Nosrati M, Dey D, Mehrani A, Strassler SE, Zelinskaya N, Hoffer ED, Stagg SM, Dunham CM, Conn GL. Functionally critical residues in the aminoglycoside resistance-associated methyltransferase RmtC play distinct roles in 30S substrate recognition. J Biol Chem. 2019 Nov 15;294(46):17642-17653. doi: 10.1074/jbc.RA119.011181. Epub 2019 Oct 8. PubMed PMID: 31594862; PubMed Central PMCID: PMC6873201.
Calderon BM, Danzy S, Delima GK, Jacobs NT, Ganti K, Hockman MR, Conn GL, Lowen AC, Steel J. Dysregulation of M segment gene expression contributes to influenza A virus host restriction. PLoS Pathog. 2019 Aug;15(8):e1007892. doi: 10.1371/journal.ppat.1007892. eCollection 2019 Aug. PubMed PMID: 31415678; PubMed Central PMCID: PMC6695095.
Conn GL, Bavro VN, Davies C. Editorial: Bacterial Mechanisms of Antibiotic Resistance: A Structural Perspective. Front Mol Biosci. 2019;6:71. doi: 10.3389/fmolb.2019.00071. eCollection 2019. PubMed PMID: 31475158; PubMed Central PMCID: PMC6702466.
Schwartz SL, Conn GL. RNA regulation of the antiviral protein 2'-5'-oligoadenylate synthetase. Wiley Interdiscip Rev RNA. 2019 Jul;10(4):e1534. doi: 10.1002/wrna.1534. Epub 2019 Apr 15. Review. PubMed PMID: 30989826; PubMed Central PMCID: PMC6585406.
Prezioso SM, Duong DM, Kuiper EG, Deng Q, Albertí S, Conn GL, Goldberg JB. Trimethylation of Elongation Factor-Tu by the Dual Thermoregulated Methyltransferase EftM Does Not Impact Its Canonical Function in Translation. Sci Rep. 2019 Mar 5;9(1):3553. doi: 10.1038/s41598-019-39331-x. PubMed PMID: 30837495; PubMed Central PMCID: PMC6401129.
Calderon BM, Conn GL. A human cellular noncoding RNA activates the antiviral protein 2'-5'-oligoadenylate synthetase 1. J Biol Chem. 2018 Oct 12;293(41):16115-16124. doi: 10.1074/jbc.RA118.004747. Epub 2018 Aug 20. PubMed PMID: 30126839; PubMed Central PMCID: PMC6187638.
Vinal K, Conn GL. Substrate Recognition and Modification by a Pathogen-Associated Aminoglycoside Resistance 16S rRNA Methyltransferase. Antimicrob Agents Chemother. 2017 May;61(5). doi: 10.1128/AAC.00077-17. Print 2017 May. PubMed PMID: 28289026; PubMed Central PMCID: PMC5404524.
Calderon BM, Conn GL. Human noncoding RNA 886 (nc886) adopts two structurally distinct conformers that are functionally opposing regulators of PKR. RNA. 2017 Apr;23(4):557-566. doi: 10.1261/rna.060269.116. Epub 2017 Jan 9. PubMed PMID: 28069888; PubMed Central PMCID: PMC5340918.
Witek MA, Kuiper EG, Minten E, Crispell EK, Conn GL. A Novel Motif for S-Adenosyl-l-methionine Binding by the Ribosomal RNA Methyltransferase TlyA from Mycobacterium tuberculosis. J Biol Chem. 2017 Feb 3;292(5):1977-1987. doi: 10.1074/jbc.M116.752659. Epub 2016 Dec 27. PubMed PMID: 28031456; PubMed Central PMCID: PMC5290967.
Fasken MB, Losh JS, Leung SW, Brutus S, Avin B, Vaught JC, Potter-Birriel J, Craig T, Conn GL, Mills-Lujan K, Corbett AH, van Hoof A. Insight into the RNA Exosome Complex Through Modeling Pontocerebellar Hypoplasia Type 1b Disease Mutations in Yeast. Genetics. 2017 Jan;205(1):221-237. doi: 10.1534/genetics.116.195917. Epub 2016 Oct 24. PubMed PMID: 27777260; PubMed Central PMCID: PMC5223504.
Kahn RA, Conn GL, Pavlath GK, Corbett AH. Use of a Grant Writing Class in Training PhD Students. Traffic. 2016 Jul;17(7):803-14. doi: 10.1111/tra.12398. Epub 2016 May 3. PubMed PMID: 27061800.
Owings JP, Kuiper EG, Prezioso SM, Meisner J, Varga JJ, Zelinskaya N, Dammer EB, Duong DM, Seyfried NT, Albertí S, Conn GL, Goldberg JB. Pseudomonas aeruginosa EftM Is a Thermoregulated Methyltransferase. J Biol Chem. 2016 Feb 12;291(7):3280-90. doi: 10.1074/jbc.M115.706853. Epub 2015 Dec 16. PubMed PMID: 26677219; PubMed Central PMCID: PMC4751374.
Witek MA, Conn GL. Functional dichotomy in the 16S rRNA (m1A1408) methyltransferase family and control of catalytic activity via a novel tryptophan mediated loop reorganization. Nucleic Acids Res. 2016 Jan 8;44(1):342-53. doi: 10.1093/nar/gkv1306. Epub 2015 Nov 24. PubMed PMID: 26609134; PubMed Central PMCID: PMC4705659.
Vachon VK, Conn GL. Adenovirus VA RNA: An essential pro-viral non-coding RNA. Virus Res. 2016 Jan 2;212:39-52. doi: 10.1016/j.virusres.2015.06.018. Epub 2015 Jun 25. Review. PubMed PMID: 26116898.
Corrêa LL, Witek MA, Zelinskaya N, Picão RC, Conn GL. Heterologous Expression and Functional Characterization of the Exogenously Acquired Aminoglycoside Resistance Methyltransferases RmtD, RmtD2, and RmtG. Antimicrob Agents Chemother. 2016 Jan;60(1):699-702. doi: 10.1128/AAC.02482-15. Print 2016 Jan. PubMed PMID: 26552988; PubMed Central PMCID: PMC4704232.
Zelinskaya N, Witek MA, Conn GL. The Pathogen-Derived Aminoglycoside Resistance 16S rRNA Methyltransferase NpmA Possesses Dual m1A1408/m1G1408 Specificity. Antimicrob Agents Chemother. 2015 Dec;59(12):7862-5. doi: 10.1128/AAC.01872-15. Epub 2015 Sep 28. PubMed PMID: 26416864; PubMed Central PMCID: PMC4649141.
Sunita S, Schwartz SL, Conn GL. The Regulatory and Kinase Domains but Not the Interdomain Linker Determine Human Double-stranded RNA-activated Kinase (PKR) Sensitivity to Inhibition by Viral Non-coding RNAs. J Biol Chem. 2015 Nov 20;290(47):28156-28165. doi: 10.1074/jbc.M115.679738. Epub 2015 Oct 2. PubMed PMID: 26432638; PubMed Central PMCID: PMC4653674.
Myers CL, Kuiper EG, Grant PC, Hernandez J, Conn GL, Honek JF. Functional roles in S-adenosyl-L-methionine binding and catalysis for active site residues of the thiostrepton resistance methyltransferase. FEBS Lett. 2015 Oct 24;589(21):3263-70. doi: 10.1016/j.febslet.2015.09.028. Epub 2015 Oct 9. PubMed PMID: 26450779; PubMed Central PMCID: PMC4661090.
Savic M, Sunita S, Zelinskaya N, Desai PM, Macmaster R, Vinal K, Conn GL. 30S Subunit-dependent activation of the Sorangium cellulosum So ce56 aminoglycoside resistance-conferring 16S rRNA methyltransferase Kmr. Antimicrob Agents Chemother. 2015 May;59(5):2807-16. doi: 10.1128/AAC.00056-15. Epub 2015 Mar 2. PubMed PMID: 25733511; PubMed Central PMCID: PMC4394793.
Vachon VK, Calderon BM, Conn GL. A novel RNA molecular signature for activation of 2'-5' oligoadenylate synthetase-1. Nucleic Acids Res. 2015 Jan;43(1):544-52. doi: 10.1093/nar/gku1289. Epub 2014 Dec 4. PubMed PMID: 25477390; PubMed Central PMCID: PMC4288181.
Hudson WH, Pickard MR, de Vera IM, Kuiper EG, Mourtada-Maarabouni M, Conn GL, Kojetin DJ, Williams GT, Ortlund EA. Conserved sequence-specific lincRNA-steroid receptor interactions drive transcriptional repression and direct cell fate. Nat Commun. 2014 Nov 7;5:5395. doi: 10.1038/ncomms6395. PubMed PMID: 25377354; PubMed Central PMCID: PMC4280027.
Kuiper EG, Conn GL. Binding induced RNA conformational changes control substrate recognition and catalysis by the thiostrepton resistance methyltransferase (Tsr). J Biol Chem. 2014 Sep 19;289(38):26189-26200. doi: 10.1074/jbc.M114.574780. Epub 2014 Aug 1. PubMed PMID: 25086036; PubMed Central PMCID: PMC4176221.
Witek MA, Conn GL. Expansion of the aminoglycoside-resistance 16S rRNA (m(1)A1408) methyltransferase family: expression and functional characterization of four hypothetical enzymes of diverse bacterial origin. Biochim Biophys Acta. 2014 Sep;1844(9):1648-55. doi: 10.1016/j.bbapap.2014.06.012. Epub 2014 Jun 22. PubMed PMID: 24963996; PubMed Central PMCID: PMC4122235.
Wilson JL, Vachon VK, Sunita S, Schwartz SL, Conn GL. Dissection of the adenoviral VA RNAI central domain structure reveals minimum requirements for RNA-mediated inhibition of PKR. J Biol Chem. 2014 Aug 15;289(33):23233-23245. doi: 10.1074/jbc.M114.550046. Epub 2014 Jun 26. PubMed PMID: 24970889; PubMed Central PMCID: PMC4132820.
Dunkle JA, Vinal K, Desai PM, Zelinskaya N, Savic M, West DM, Conn GL, Dunham CM. Molecular recognition and modification of the 30S ribosome by the aminoglycoside-resistance methyltransferase NpmA. Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6275-80. doi: 10.1073/pnas.1402789111. Epub 2014 Apr 9. PubMed PMID: 24717845; PubMed Central PMCID: PMC4035980.
Linpinsel JL, Conn GL. General protocols for preparation of plasmid DNA template, RNA in vitro transcription, and RNA purification by denaturing PAGE. Methods Mol Biol. 2012;941:43-58. doi: 10.1007/978-1-62703-113-4_4. PubMed PMID: 23065552.
Avis JM, Conn GL, Walker SC. Cis-acting ribozymes for the production of RNA in vitro transcripts with defined 5' and 3' ends. Methods Mol Biol. 2012;941:83-98. doi: 10.1007/978-1-62703-113-4_7. PubMed PMID: 23065555.
Vachon VK, Conn GL. Plasmid template design and in vitro transcription of short RNAs within a "structure cassette" for structure probing experiments. Methods Mol Biol. 2012;941:157-69. doi: 10.1007/978-1-62703-113-4_12. PubMed PMID: 23065560.
Bryk R, Wu K, Raimundo BC, Boardman PE, Chao P, Conn GL, Anderson E, Cole JL, Duffy NP, Nathan C, Griffin JH. Identification of new inhibitors of protein kinase R guided by statistical modeling. Bioorg Med Chem Lett. 2011 Jul 1;21(13):4108-14. doi: 10.1016/j.bmcl.2011.04.149. Epub 2011 May 13. PubMed PMID: 21632247.
Templeton CM, Ostovar pour S, Hobbs JR, Blanch EW, Munger SD, Conn GL. Reduced sweetness of a monellin (MNEI) mutant results from increased protein flexibility and disruption of a distant poly-(L-proline) II helix. Chem Senses. 2011 Jun;36(5):425-34. doi: 10.1093/chemse/bjr007. Epub 2011 Feb 22. PubMed PMID: 21343241; PubMed Central PMCID: PMC3094690.
Zelinskaya N, Rankin CR, Macmaster R, Savic M, Conn GL. Expression, purification and crystallization of adenosine 1408 aminoglycoside-resistance rRNA methyltransferases for structural studies. Protein Expr Purif. 2011 Jan;75(1):89-94. doi: 10.1016/j.pep.2010.07.005. Epub 2010 Jul 25. PubMed PMID: 20667473; PubMed Central PMCID: PMC2966526.
Macmaster R, Zelinskaya N, Savic M, Rankin CR, Conn GL. Structural insights into the function of aminoglycoside-resistance A1408 16S rRNA methyltransferases from antibiotic-producing and human pathogenic bacteria. Nucleic Acids Res. 2010 Nov;38(21):7791-9. doi: 10.1093/nar/gkq627. Epub 2010 Jul 17. PubMed PMID: 20639535; PubMed Central PMCID: PMC2995053.
Launer-Felty K, Wong CJ, Wahid AM, Conn GL, Cole JL. Magnesium-dependent interaction of PKR with adenovirus VAI. J Mol Biol. 2010 Oct 1;402(4):638-44. doi: 10.1016/j.jmb.2010.08.015. Epub 2010 Aug 14. PubMed PMID: 20713064; PubMed Central PMCID: PMC2980848.
Moric I, Bajkic S, Savic M, Tomic TI, Conn GL, Vasiljevic B. Heterologous Escherichia coli expression, purification and characterization of the GrmA aminoglycoside-resistance methyltransferase. Protein J. 2009 Oct;28(7-8):326-32. doi: 10.1007/s10930-009-9197-9. PubMed PMID: 19763405.
Wahid AM, Coventry VK, Conn GL. The PKR-binding domain of adenovirus VA RNAI exists as a mixture of two functionally non-equivalent structures. Nucleic Acids Res. 2009 Sep;37(17):5830-7. doi: 10.1093/nar/gkp595. Epub 2009 Jul 27. PubMed PMID: 19635730; PubMed Central PMCID: PMC2761268.
Savic M, Lovric J, Tomic TI, Vasiljevic B, Conn GL. Determination of the target nucleosides for members of two families of 16S rRNA methyltransferases that confer resistance to partially overlapping groups of aminoglycoside antibiotics. Nucleic Acids Res. 2009 Sep;37(16):5420-31. doi: 10.1093/nar/gkp575. Epub 2009 Jul 9. PubMed PMID: 19589804; PubMed Central PMCID: PMC2760815.
Dunstan MS, Hang PC, Zelinskaya NV, Honek JF, Conn GL. Structure of the thiostrepton resistance methyltransferase.S-adenosyl-L-methionine complex and its interaction with ribosomal RNA. J Biol Chem. 2009 Jun 19;284(25):17013-17020. doi: 10.1074/jbc.M901618200. Epub 2009 Apr 15. PubMed PMID: 19369248; PubMed Central PMCID: PMC2719339.
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